complex of cytoplasmic dynein using nmr and mass spectrometry
Báo cáo khoa học: Systematic quantification of complex metabolic flux networks using stable isotopes and mass spectrometry pptx
... name of an amino acid in the legend refers to the weight of the depicted fragment ion of the TBDMS-derivative of the amino acid of 18 GC-MS runs and its standard deviation reflects the variance of ... measured mass isotopomer distributions of Glu, fragment [2-4] of Asp and fragment 2-3 of Ala not contain any gross errors, then the estimated (from Eqn 1) and measured mass isotopomer distribution of ... concentration of valine was at the limits of the detection method (HPLC) The high standard deviations assigned to CER, OUR and the net consumption rate of ammonia (i.e 10%, 10% and 15% of the rate...
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Tài liệu Báo cáo khóa học: Quantitative analysis, using MALDI-TOF mass spectrometry, of the N-terminal hydrolysis and cyclization reactions of the activation 2 process of onconase pdf
... reducing it once purified By using the purified recombinant protein, the conversion of reagents and products of the exopeptidasecatalyzed hydrolysis of Met1 and cyclization of Gln1 to pyroglutamyl ... mass 11 818.87) and Pyr-ONC (M23L) (theoretical molecular mass 11 801.60), and expressing it as the fraction of hydrolyzed (Met1)-ONC (M23L) Figure shows the results of the characterization of ... on cyclization of the amino terminal glutamine residues of tryptic peptides of streptococcal PepM49 protein Resolution of cyclized peptides by HPLC and characterization by mass spectrometry Int...
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Structural investigation of silicon nanowires using GIXD and GISAXS evidence of complex saw tooth faceting
... respectively the real and measured angles between ~ and the vertical and u the angle between the normal of the facet and the q CCD plane tions of Ross et al in [5] which show that only one out of two sides ... sidewall morphology and Ross et al [5] with saw-tooth faceting They interpret this faceting term of both the role of the geometry and surface energy of the wire and the liquid droplet, and report that ... provide evidence of the hexagonal cross-section of the wires The angle between two streaks is 60° and the directions of the six sides of the nanowires can be deduced from the directions of these streaks...
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Báo cáo hóa học: " Efficient Implementation of Complex Modulated Filter Banks Using Cosine and Sine Modulated Filter Banks" pot
... of with and without a unit delay The critical sampling is obtained by taking the real part of one polyphase component and the imaginary part of the other polyphase component in each subband and ... is realized using K cascaded butterfly stages and pure delays Each butterfly stage consists of M/2 butterflies that are realized by using four multipliers and two adders The number of multiplications ... COMPLEXITY OF EMFBs By using the algorithm presented in [15], the lowest computational complexities of DCT-IV and DST-IV are μ(M) = (M/2) log2 M + M and α(M) = (3M/2) log2 M, where the μ(M) and α(M)...
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Báo cáo sinh học: "High-resolution quantitative imaging of mammalian and bacterial cells using stable isotope mass spectrometry" docx
... 13C- and 12C1H- (mass 13.003355 and mass 13.007825, respectively; Figure 15b); 12C14N- and 1H12C13C (mass 26.003074 and mass 26.011180, respectively; Figure 15c); 12C15N-, 13C14N- and 12C14N1H- (mass ... Figure 2b, and the mass image of the 12C14N- ions sputtered from a 20-m subfield of Figure 2b is shown in Figure 2c The contrast of these mass images provides a very detailed view of the cochlear ... and uniform carbon content The image of the 13C- ions (Figure 3e) is similar to the 12Cimage, but with a much lower count rate; the total number of counts of 13C- and of 12C- are 2.56 x 105 and...
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Báo cáo y học: "Psoriasin, one of several new proteins identified in nasal lavage fluid from allergic and non-allergic individuals using 2-dimensional gel electrophoresis and mass spectrometry" potx
... history of allergic rhinitis and had negative SPT to the standard panel of allergens as described above They had no history of upper airway infection for weeks before the time of visit and they ... were loaded in a Micromass M@ldi MALDI-TOF mass spectrometer (Waters, Milford, USA) for analysis of the peptide masses Database search Peptide masses retrieved from MALDI-TOF analysis spectra were ... allergic rhinitis and 11 healthy volunteers (7 women), serving as controls The mean age of patients and controls was 43 (26–55) and 41 (24–55) years, respectively The diagnosis of birch and/ or grass...
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Báo cáo y học: "Quality evaluation of mycelial Antrodia camphorata using high-performance liquid chromatography (HPLC) coupled with diode array detector and mass spectrometry (DAD-MS)" pps
... array detector and mass spectrometry (HPLCDAD-ESI-MS) and to assess the quality of two commercial mycelial AC products Methods Plant Powdered mycelium and an intact fruiting body of AC were supplied ... the full profile of nucleosides and nucleobases in AC can be useful in developing a fingerprint An extensive determination of the nucleoside and nucleobase profiles in the water extract of AC was ... chromatographic profile of the water extract of mycelial AC Comprehensive chemical profile of AC The appropriate solvent should be used to extract as many groups of representative chemical classes and compounds...
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Báo cáo khoa học: A region within the C-terminal domain of Ure2p is shown to interact with the molecular chaperone Ssa1p by the use of cross-linkers and mass spectrometry doc
... nondeuterated and deuterated cross-linkers by MALDI-TOF-TOF mass spectrometry A selection of mass spectra illustrates how the comparison of chymotryptic peptides from different cross-linked complexes and ... (A) MALDI-TOF-TOF mass spectrum of the light (d0) and heavy (d4) precursor ions presenting a mass difference of 4.024 Da (indicated by the curved arrow) (B) Fragmentation mass spectrum of the double-charged ... citrate and 0.1% formic acid) and spotted (0.5 lL) on a stainless steel MALDI target (Opti-TOF; Applied Biosystems) MALDI-TOF-TOF MS and MS ⁄ MS spectra were acquired with a MALDI-TOF ⁄ TOFÔ 4800 mass...
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Báo cáo khoa học: Interaction of the C1 complex of Complement with sulfated polysaccharide and DNA probed by single molecule fluorescence microscopy pdf
... site of interaction on the protein For this purpose we took advantage of the binding properties of C1q toward DNA and of an emerging technique allowing the molecular combing of DNA strands and ... Actually the binding of the C-reactive protein [20], of the serum amyloid protein [21] and of DNA [22] to C1q leads to an activation of Complement On the other hand, the binding of sulfated glycosaminoglycans ... mixed with 10 lL of labeled purified C1q One hundred and fifty microliters of 6.5 pM T4 DNA were mixed with lL of labeled CLR or 2.5 lL of labeled C1, and according to case with 1–2 lL of 45 lM fucoidan...
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Báo cáo khoa học: Spectroscopic characterization of the oxyferrous complex of prostacyclin synthase in solution and in trapped sol–gel matrix doc
... diffusion of the substrate We also estimated the enzyme activities of aqueous PGIS and encapsulated PGIS using the same concentration of guaiacol and peracetic acid The initial rates of the aqueous and ... the intensity of the a band (556 nm) was greater than that of the b band (530 nm) This spectral feature is similar to that of oxyferrous complexes of P450s, particularly at the Q band region in ... oxygen, an absorption spectrum of the re-oxidized sample showed the features of the resting enzyme (i.e a Soret peak at 418 nm and discrete a bands and b bands at 570 and 537 nm, respectively (data...
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foundations of 3d graphics using jogl and java3d
... OpenGL command, we often omit the interface handle Here we set the physical size of frame and make its contents visible Here the physical size corresponds to the number of pixels in x and y direction ... Scan-conversion, and Clipping generate a random line with -1
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ECE 574: Modeling and Synthesis of Digital Systems using Verilog and VHDL ppsx
... Applications of LFSRs include generating pseudo-random numbers, pseudo-noise sequences, fast digital counters, and whitening sequences Both hardware and software implementations of LFSRs are ... count from to 77 on a negative edge of the clock and then restart at ECE574: VHDL and Verilog Modeling and Synthesis 12/08/10 Question [20 marks]: An FPGA running off its own local 100MHz oscillator ... after an access time of tacc from OE and CE Write operation (CE and WE valid - write data on the rising edge of WE) In the model check that the address bus is stable for taddr and the data bus is...
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Báo cáo nghiên cứu khoa học: " BUILDING A GOOD IMAGE FROM TWO COLOR IMAGES OF CAMERA MODEL USING GA AND DISCRETE WAVELET TRANSFORM" pptx
... sets of data before fusing and forming a new set of data DWT is one of popular tools used in MRA by using low-pass and high-pass filter [6, 7, 8] Consider is DWT performance, the analysis and ... 10 Histogram and standard deviation comparisons (a), (c), (e) are histograms and standard deviation corresponding to R, G, B color of image 9a; (b), (d), (f) are histograms and standard deviation ... 11 Histogram and standard deviation comparisons (a), (c), (e) are histograms and standard deviation corresponding to R, G, B color of image 9b; (b), (d), (f) are histograms and standard deviation...
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Báo cáo y học: "Proteomic profiling of the mesenteric lymph after hemorrhagic shock: Differential gel electrophoresis and mass spectrometry analysis" pdf
... 50% ACN and 1.0% FA Mass Spectrometry Matrix-assisted laser desorption ionization (MALDI) tandem time -of- flight (TOF/TOF) mass spectrometry was carried out on an Applied Biosystems 4700 mass spectrometer, ... digested and identified by mass spectrometry Along with the 154 spots, 12 additional spots were selected as prominent features of the gel, and were added to the list of potential proteins of interest ... extraction, in-gel proteolytic digestion and identification of proteins via tandem mass spectrometry analysis Here, we measured the proteomic profile of mesenteric lymph to identify underlying...
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Báo cáo y học: "Comparison of raw and processed Radix Polygoni Multiflori (Heshouwu) by high performance liquid chromatography and mass spectrometry" pptx
... employed to determine the level of anthraquinones in Radix Polygoni Multiflori [32,33] Using HPLC-DAD and mass spectrometry, the present study compares five pairs of raw and processed Radix Polygoni ... were used for preparation of the mobile phase Analytical grade methanol (Labscan, Thailand) was used for preparation of standards and sample extraction Reference compounds of 2,3,5,4′-tetrahydroxystilbene-2-O-b-D- ... of Figure HPLC fingerprints of R-RPM and its corresponding P-RPM from various sources in China Table MS data of major identified/unknown compounds in the HPLC chromatograms of R-RPM Peak No Mass...
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MODELING OF OPENCAST MINES USING SURPAC AND ITS OPTIMIZATION
... work and had also made a comparative study on which module of particular software is user friendly and easy to learn and the accuracy of results obtained from them A detailed review of mining softwares ... ࢙࢚ࢇ࢚࢛࢙ሺ࢚ሻ, 1≤i≤N and 1≤j≤M (1) where, National Institute of Technology, Rourkela-769008 Page 20 Si(t) = demand of shovel I for dumpers Θij(t) = ability of dumper j to respond to the demand of shovel ... Manno, Switzerland Ronson K.A, 2001, Computerized open pit planning and the development and application of a software open pit planner Sattarvand J, Delius C.N, 2008, Perspective of meta-heuristic...
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Functions of cytoplasmic dynein in drosophila epithelia cells
... intermediate and light chains (Fig 1.5) (Asai and Wilkes, 2004) The fine structure of cytoplasmic dynein, the force generation and the regulation of dynein are not within the scope of this study, ... endosomes in dynein mutant 115 Fig Dynein functions between S2 and S3 cleavage of Notch (1) 118 Fig 10 Dynein functions between S2 and S3 cleavage of Notch (2) 119 IX Fig 11 Dynein is recruited ... CRB2 and CRB3 (den Hollander et al., 1999) Mammalian CRB1 is the human ortholog of Drosophila Crb, and is expressed in the eye and brain CRB1 and CRB2 show conserved domain architecture of both...
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Identifying protein co factors of oct4, an essential stemness transcription factor, by affinity purification and mass spectrometry
... proteome of ES cells, by comparative analysis of pluripotent to differentiated ES cells (Baharvand et al., 2008) , the use of microscale twodimensional liquid chromatography and tandem mass spectrometry ... Basis of Affinity Purification 100 Tandem Affinity Purifications 117 Preparing Proteins for Mass Spectrometry 121 5.1 Identification of Purified Proteins by Mass Spectrometry ... mechanism of DNA methylation in ES cells, the loss of Dnmt3a and Dnmt3b also resulted in a loss of methylation at the key promoters of Pou5f1 and Nanog examined (Li et al., 2007), and a concomitant...
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analysis of proteins and protein complexes by chemical cross linking and mass spectrometry
... proteomics: Analysis of proteins and protein complexes by chemical cross-linking and mass spectrometry Zhuo A Chen Thesis for the Degree of Doctor of Philosophy The University of Edinburgh August ... complex samples were mainly conducted on MALDI-TOF, MALDI-TOF/TOF, ESI-TOF (electrospray ionization-TOF) and ESI-Q-TOF (ESI-linear trap quadrupole-TOF) instruments (Sinz, 2006; Jin Lee, 2008; Leitner ... techniques 1.1.2 Applications of mass spectrometry in protein structural analysis Today mass spectrometry plays important roles in structural biology studies Mass spectrometry based proteomics...
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